Plant Cell Culture & Micropropagation - ISSN 1808-9909 http://pccm.ufla.br/index.php/plantcellculturemicropropagation <p><strong>Plant Cell Culture &amp; Micropropagation</strong> is a journal that publishes scientific papers in the area of plant tissue culture and applied plant biotechnology, including micropropagation; genetic transformation; plant regeneration; organogenesis, and somatic embryogenesis, morphogenesis; functional genomics and metabolic engineering.</p><p><span>Submitted manuscripts must be written in English, be original, and be in accordance with the journal's standards and not be submitted for publication elsewhere. Its content (data, ideas, opinions, and concepts) is the sole responsibility of the author(s). </span></p><p><strong><span style="font-size: 1.17em;"><br /></span></strong></p><p><strong><span style="font-size: 1.17em;">ONLINE SUBMISSIONS</span></strong></p><p>Already have a Username/Password for Plant Cell Culture &amp; Micropropagation?<br /><a class="action" href="/ojs/index.php/PlantCellCultureMicropropagation/login">GO TO LOGIN</a></p><p>Need a Username/Password?<br /><a class="action" href="/ojs/index.php/PlantCellCultureMicropropagation/user/register">GO TO REGISTRATION</a></p><p>Registration and login are required to submit items online and to check the status of current submissions.</p><p><span><br /></span></p><p><span style="font-size: 1.17em;"><strong>AUTHOR GUIDELINES</strong> (<a href="/ojs/index.php/PlantCellCultureMicropropagation/about/submissions#authorGuidelines">http://177.105.2.193/ojs/index.php/PlantCellCultureMicropropagation/about/submissions#authorGuidelines</a>)</span></p><p> </p><p> </p><p><strong>INFORMATION</strong></p><p><strong>PREZADOS USUÁRIOS AO SE CADASTRAREM NO SISTEMA VERIFIQUEM SE TODOS OS DADOS FORAM PREENCHIDOS E NA OPÇÃO URL ADICIONEM O LINK PARA O CURRÍCULO LATTES. </strong></p><p><strong>É IMPORTANTE QUE TANTO OS AUTORES COMO OS CO-AUTORES SE CADASTREM NO SISTEMA NO MOMENTO DA SUBMISSÃO DE ARTIGOS.</strong></p> en-US plantcellcm@gmail.com (Plant Cell Culture & Micropropagation) plantcellcm@gmail.com (Equipe Editorial) Sat, 10 Oct 2015 00:00:00 -0300 OJS 3.2.1.1 http://blogs.law.harvard.edu/tech/rss 60 SOMATIC EMBRYOGENESIS IN MAIZE: BACKGROUND AND EFFICIENCY http://pccm.ufla.br/index.php/plantcellculturemicropropagation/article/view/8 <p>The selection of efficient protocols to promote somatic embryogenesis and plant regeneration of maize (<em>Zea mays </em>L.), is a target that should contribute to the improvement of maize in Brazil. This process should encourage the transfer of genes and the development of genetically modified varieties. In the present review, protocols have been organized and developed for embryogenic callus induction and plant regeneration in order to evaluate the efficiency of the protocols that were used. One or more promising protocols for future investments was selected with genotypes of interest for genetic improvement. The investigations with different maize genotypes are concentrated in just over two decades (1989-2012), when only 17 studies were published and 179 genotypes were investigated, of which 77% formed embryogenic callus and only 43.5% regenerated plants in the absence or presence of amino acids and/or in different concentration of cytokinins and auxins. The N6 medium was the most used for callus induction (89.47% of investigations). The addition of L-proline, hydrolyzed casein, along with AgNO3 was detected in 84, 56, and 60% of the experiments, respectively. N6 medium containing 2.0 mg L-1 2.4-D has been used to analyze 46% of the genotypes. Through this process somatic embryogenesis has been induced in 87.9% of these cases. It has been three decades since the release of the first corn regenerant (1975), the competence for somatic embryogenesis and plant regeneration has been linked to being more dependent on genotype than on added supplements to the culture medium.</p> ANA LUISA MONEZI LUCENA, IVONE BATISTA DE OLIVEIRA ELOI, CLAUDETE APARECIDA MANGOLIN, MARIA DE FÁTIMA PIRES DA SILVA MACHADO Copyright (c) 2016 Plant Cell Culture & Micropropagation http://pccm.ufla.br/index.php/plantcellculturemicropropagation/article/view/8 Fri, 29 May 2015 00:00:00 -0300 CARBON SOURCE ON in vitro MULTIPLICATION OF ‘MC’ AND ‘ADAMS’ QUINCE ROOTSTOCKS http://pccm.ufla.br/index.php/plantcellculturemicropropagation/article/view/10 <p>The objective was to determine the best carbon source and<br />concentration for in vitro multiplication of quince MC and Adams<br />cultivars. Nodal segments (1.0 cm and 1-2 buds) were inoculated<br />in MS and as a carbon source, sucrose, fructose or sorbitol were<br />used with the following concentrations: 0, 15, 30, 45 and 60 g L-1.<br />After 60 days, it was observed that, for ‘MC’, 60 g L-1 sucrose and<br />30 g L -1 fructose yielded 100% sprouted explants. The highest<br />shoot length average (1.49 cm) was obtained with 30 g L-1 sucrose<br />and 60 g L-1 sorbitol. The concentration of 45 g L-1 provided the<br />highest number of shoots (1.76), higher fresh mass (0.058 g) and higher dry matter (0.02 g), regardless of the carbon source. For ‘Adams’, the highest number of shoots (1.47) was obtained with sucrose and the higher dry mass of shoots (0.01 g) was obtained with sucrose and sorbitol. The sucrose concentration of 30 g L-1 provided the highest shoot lenght average (2.14 cm) and higher fresh weight (0.1 g) and 45 g L-1 sorbitol provided greater dry mass of shoots (0.03 g ). The highest percentage of sprouted explants was obtained at a concentration of 30 g L-1 (79.58%) and sucrose as carbon source (71.25%). The highest average number of shoots (1.45) was obtained with 30 g L-1. It was concluded that 45 g L-1 sucrose for ‘MC’ and 30 g L-1 sucrose for ‘Adams’ were the best concentrations for in vitro multiplication.</p> MIRIAN DE FARIAS RIBEIRO, CRISTINA WEISER RITTERBUSCH, VALMOR JOÃO BIANCHI, JOSÉ ANTONIO PETERS Copyright (c) 2016 Plant Cell Culture & Micropropagation http://pccm.ufla.br/index.php/plantcellculturemicropropagation/article/view/10 Fri, 23 Oct 2015 00:00:00 -0200 In vitro GERMINATION AND ACCLIMATIZATION OF EVERLASTING FLOWER http://pccm.ufla.br/index.php/plantcellculturemicropropagation/article/view/9 <p>Actinocephalus bongardii (A. St.-Hil.) Sano is a native<br />ornamental specie from Brazil, popularly known as evergreen<br />“showerhead” that presents potential for commercialization.<br />No effective propagation protocol for the species has yet been<br />established. The objective of this work was to establish an in vitro<br />germination protocol and acclimatization of A. bongardii. For the<br />in vitro culture, we evaluated MS and WPM culture media. Three<br />pH levels (4.8, 5.8 and 6.8) and five concentrations of gibberellic<br />acid (GA3) (0.0; 0.2; 0.4; 0.6 and 0.8 mg L-1) in WPM culture<br />media were evaluated. Percentage of germinated seeds, number<br />of leaves and length of the seedlings were evaluated 30 days after<br />inoculation. Seedlings obtained through the in vitro germination<br />were acclimatized with Plantmax® substrate in a greenhouse and<br />the percentage of seedlings survival were evaluated 30 days after acclimatization. The seeds inoculated in WPM showed highest germination percentage (74%) and seedlings with more leaves and longer length. The culture medium pH of 5.8 improved germination and the supplementation of the medium with 0.2 mg L-1 GA3 significantly increased the percentage of seed germination (94%). The acclimatization of the seedlings was only 50% due to the fragility of the tissues.</p> DÉBORA DE OLIVEIRA PRUDENTE, Fernanda Carlota Nery, MICHELE VALQUÍRIA DOS REIS, PATRÍCIA DUARTE DE OLIVEIRA PAIVA, MARCELA CARLOTA NERY, THAÍS DE OLIVEIRA AMIN Copyright (c) 2016 Plant Cell Culture & Micropropagation http://pccm.ufla.br/index.php/plantcellculturemicropropagation/article/view/9 Thu, 31 Dec 2015 00:00:00 -0200 MULTIPLICATION AND in vitro ROOTING OF Campomanesia adamantium CAMB. http://pccm.ufla.br/index.php/plantcellculturemicropropagation/article/view/11 <p>The aim of this work was to evaluate the influence of<br />growth regulators BAP, IBA, NAA and IAA in the propagation<br />of Campomanesia adamantium CAMB. Nodal segments<br />from seedlings maintained in vitro at the fourth subculture<br />(subculture 40 to 40 days) were used. WPM culture medium </p><p>supplemented with 30 g L-1 sucrose, 7 g L-1 agar and 0.9 mM<br />PVP was used. For shoot induction, different concentrations<br />(0; 0.25; 0.5; 1.0 and 2.0 mg L-1) of BAP were tested. For<br />root induction, different concentrations (0.5; 1.0; 1.5 and 2.0<br />mg L-1) of IBA, NAA and IAA were used. The explants were<br />maintained in growth room with light intensity of 43 μmol<br />m-2 s-1 and a temperature of 25 ºC. We used a completely<br />randomized design with 5 replicates (each replicate with<br />three tubes). For shoot length, analysis of the experiment<br />was conducted in a factorial 2x5 (two evironments and five<br />concentrations). For the shoot variable, multiplication rate<br />and leaves presented no significant difference, but the length<br />shoot showed the highest average using 1.0 mg L-1 BAP<br />when induced in the dark. Results for root number were not<br />significant. The concentration indicated for shoot induction<br />is 1.0 mg L-1 BAP maintained in the dark for elongation. No<br />efficient protocol for root formation was obtained.</p> MARIELI ROSSATO, PEDRO VITOR SCHUMACHER, ANTÔNIO PAULINO DA COSTA NETTO, GEICIANE CINTRA DE SOUZA, EDÉSIO FIALHO DOS REIS, VANESSA CRISTINA STEIN Copyright (c) 2016 Plant Cell Culture & Micropropagation http://pccm.ufla.br/index.php/plantcellculturemicropropagation/article/view/11 Thu, 31 Dec 2015 00:00:00 -0200